Advanced Applications of Fluorescence Techniques

In the beginning of July Anna Stanczak (ERS5) traveled to Italy to participate in ‘Advanced Application of Fluorescence Techniques’ course. The course was organised by Fluorescence Foundation the educational branch of ISS and was hosted by the Institute of Food Sciences of National Research Council of Italy.

The 3 days course contained both theoretical and practical part. Lectures were presented by the experts in the field and covered a broad range of topics from the basis of fluorescence phenomena to state-of-art imaging of single molecule. The theoretical part was also complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications.

The full list of topics covered by the course is presented below:

  • Basic Definitions and Principles of Fluorescence
  • Fluorescence Polarization
  • Time-resolved Fluorescence
  • Instrumentation
  • Data Manipulation and Data Analysis
  • Fluorescence Probes
  • Confocal and Multiphoton Fluorescence Microscopy
  • FFS, Fluorescence Fluctuation Spectroscopy
  • FLIM, Fluorescence Lifetime Imaging
  • Single Molecule Imaging

I would like to thank the Fluorescence Foundation and all of the instructors for organizing such a comprehensive course, I definitely learnt a lot and had new ideas about fluorescence applications.